anti mouse pmk2  (Cell Signaling Technology Inc)

Journal: Cancer research

Article Title: Inhibition of the stromal p38MAPK/MK2 pathway limits breast cancer metastases and chemotherapy-induced bone loss

doi: 10.1158/0008-5472.CAN-18-0234

Figure Lengend Snippet: (A,B) Representative images of primary breast lesions, n=53, and bone metastatic biopsies, n=33, Scale= 15 µm (Inset scale = 100µm). Serial sections were stained with antibodies against IL-6 (A) or phosphorylated MK2 (pMK2) (B), along with hematoxylin and eosin (H&E), E-cadherin, or pan-cytokeratin (C) IHC for IL-6 in tumor versus stroma on primary breast and patient-matched bone metastatic lesions. Semi-quantitative analysis using histoscore (H-score) system. Two-tailed Wilcoxon signed-rank test, **** p<0.0001. D) IL-6 expression across molecular subtypes of breast cancer. Two-way ANOVA, ***p≤ 0.001. (E) IL-6 expression in tumor stroma versus tumor epithelium (epi) in the pan-Breast cancer dataset. Boxplot, t-test, **** p< 2.2e-16. Below the boxplot is a list of p38MAPK-dependent stromal factors expressed in the three datasets analyzed. (F) GSVA analysis for enrichment of p38MAPK-dependent protumorigenic factors in stroma and epi from breast tumor samples (right). Gene list with overlapping genes (bottom left). Significance was determined by comparing the GSVA enrichment scores of stroma versus epi within each signature, one-way ANOVA with Tukey post-hoc test, ***p≤ 0.0001. All data are displayed as mean±SEM.

Article Snippet: Slides were stained with the following antibodies: anti-IL-6 primary antibody (ab6672, 1:100, AbCam), pMK2 primary antibody (3007, 1:50, Cell Signaling), Total p38 primary antibody (9212, 1:100, Cell Signaling), Biotinylated Donkey anti-Rabbit IgG (H+L) cross-adsorbed secondary antibody (Cat#:31821, 1:500, 2.2μg/ml, Thermofisher).

Techniques: Staining, Two Tailed Test, Expressing

Journal: International journal of cancer

Article Title: Inhibition of MK2 suppresses IL-1β, IL-6, and TNF-α dependent colorectal cancer growth

doi: 10.1002/ijc.31191

Figure Lengend Snippet: A) MK2 is constitutively activated in CT26 cells. Serum-starvation (SS) reduces pMK2, but treatment with IL-1β, IL-6, or TNF-α induces MK2 phosphorylation. Intratumoral treatment with IL-1β, IL-6, and TNF-α at levels produced by vehicle tumors resulted in B) representative images of tumors with C) restored tumor size and D) weight. In organ culture supernatants, cytokine treatment increased the MK2i tumor production of E) IL-1β, F) IL-6, and G) TNF-α. Increases were seen in mRNA levels for H) IL-1β, I) IL-6, and J) TNF-α were also seen. N=10 *p < 0.05, **p<0.01, ***p < 0.005.

Article Snippet: Tumor samples were labeled by immunohistochemistry for pMK2 Thr334 (Cell Signaling Technology, Danvers, MA).

Techniques: Produced, Organ Culture